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Protein Purification Process Development |
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Avid Bioservices' team has significant expertise in the purification of recombinant proteins and antibodies. If your current purification process gives you less than desirable results, our team can assist with process optimization. Significant improvements in recovery rates and stability of purified proteins and antibodies can be achieved with process optimization.
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Our experienced staff can provide assistance for process development, process refinement, in scale-up and in scale-down. The Avid Staff is proficient in the operation of instrumentation such as the Amersham Pharmacia Biotech AKTA Explorer, Beckman HPLC and CE Systems, Light Scattering, Glyco Carbohydrate Analysis System, as well as a multitude of assays designed to provide potency and stability data all along the development pathway.
Process refinement is available to those customers that require or desire only minor changes in their current process plan. Existing methods can be manipulated to the specific needs of the project or completely engineered. Avid Bioservices is able to take your project from a T-flask scale to a 1,000L bioreactor and back again to assess any single purification issue all the way to the entire scheme. Process development and formulation optimization can provide significant improvements in actual protein expression, recovery rates and stability, ultimately saving our customers costly redevelopment and delay to market time.
We utilize appropriate scale-down systems for investigating possible improvements to the purification process including some of the following systems:
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Scaled down chromatography columns
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HPLC System Gold with UV detector
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HPLC System Gold with photodiode detector to investigate protein purification
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Carbohydrate system for investigating sialation
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Beckman P/ACE Capillary Electrophoresis
AKTA FPLC for scouting applicability of columns, buffers, pH and conductivity with simultaneous evaluation of up to eight columns.
